转录因子USF1调控鸡小肠上皮细胞中糖类转运蛋白表达

doi:10.11843/j.issn.0366-6964.2015.10.003

Abstract

Abstract:

In order to study the potential regulation mechanism of the USF1 on monosaccharide transporters in chicken intestinal epithelial cells，we built the eukaryote expression vector pcDNA3.1-USF1 on the basis of bioinformatics analysis and made the transcription factor USF1 over-expressed in chicken intestinal epithelial cells.Besides，we detected and analyzed the influence of USF1 on the mRNA expressions of SGLT1，GLUT2 and GLUT5 by Real-time PCR.The results indicated that the over-expression of USF1 gene significantly up-regulated the mRNA expressions of GLUT2 and SGLT1 in chicken intestinal epithelial cells(P＜0.01)，while the expression of GLUT5 were not changed significantly(P＞0.05).Combining the results with the bioinformatics analysis of the USF1 binding sites on the 5′up-stream regulation area of the 3 monosaccharide transporters，our results indicated that the transcription factor USF1 positively regulated the expression of SGLT1 and GLUT2 in chicken intestinal epithelial cells.

CLC Number:

S831.2

ZHANG Li-huan，LI Ling-xiang，ZHANG Yu，YUAN Yi-tong，WANG Wen-kui，ZHU Zhi-wei，WANG Qin-de，LI Hui-feng. Transcription Factor USF1 Regulate the Expression of Monosaccharide Transporters in Chicken Intestinal Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1713-1720.

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Transcription Factor USF1 Regulate the Expression of Monosaccharide Transporters in Chicken Intestinal Epithelial Cells

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